Rabbit Anti-HSD11B2 antibody

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Corticosteroid 11 beta dehydrogenase isozyme 2; 11 beta HSD2; 11 beta hydroxysteroid dehydrogenase type 2; 11 DH2; AME; AME1; HSD11K; HSD2; Hydroxysteroid 11 beta dehydrogenase 2; Hydroxysteroid 11 beta dehydrogenase isoenzyme 2; NAD dependent 11 beta hyd

Cat:

SL20186R

Species Reactivity：

Human,Mouse,Rat,(predicted: Dog,Horse,)

Immunogen：

KLH conjugated synthetic peptide derived from human HSD11B2:151-250/405

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000IF=1:100-500not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Mouse Liver tissue lysates Lane 2: Rat Stomach tissue lysatesPrimary: Anti- HSD11B2 (SL20186R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 45 kDaObserved band size: 45 kDaBlank control（black line）:MCF-7. Primary Antibody (green line): Rabbit Anti-HSD11B2 antibody ( SL20186R ) Dilution:1ug/Test; Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488Dilution: 0.5ug/Test. Isotype control（orange line）: Normal Rabbit IgGProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]

Function:
Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids.

Subunit:
Interacts with ligand-free cytoplasmic NR3C2.

Subcellular Location:
Microsome. Endoplasmic reticulum.

Tissue Specificity:
Found in placenta, kidney, pancreas, prostate, ovary, small intestine and colon.

Similarity:
Belongs to the short-chain dehydrogenases/reductases (SDR) family.

SWISS:
P80365

Gene ID:
3291

Database links:

Entrez Gene: 282434 Cow

Entrez Gene: 3291 Human

Entrez Gene: 15484 Mouse

Entrez Gene: 396948 Pig

Entrez Gene: 25117 Rat

Omim: 23630 Human

SwissProt: O77667 Cow

SwissProt: P80365 Human

SwissProt: P51661 Mouse

SwissProt: P51976 Rabbit

SwissProt: P50233 Rat

SwissProt: P50168 Sheep

Unigene: 1376 Human

Unigene: 5079 Mouse

Unigene: 10186 Rat

Picture

Blank control（black line）:MCF-7.
Primary Antibody (green line): Rabbit Anti-HSD11B2 antibody ( SL20186R )
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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