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Home > Product > Antibody > Rabbit Anti-UAP56 antibody
56 kDa U2AF65-associated protein; ATP-dependent RNA helicase p47; B(0,+)-type amino acid transporter 1; BAT1; Bat1a; DEAD box protein UAP56; Glycoprotein-associated amino acid transporter b0,+AT1; HLA-B-associated transcript 1 protein; HLA-B-associated tr
Cat:
SL20587R
Species Reactivity：
Human,Mouse,Rat,(predicted: Pig,Cow,)
Immunogen：
KLH conjugated synthetic peptide derived from human UAP56:1-100/428
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Polyclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ugLane 2: Heart (Mouse) Lysate at 40 ugLane 3: Cerebrum (Rat) Lysate at 40 ugLane 4: Liver (Rat) Lysate at 40 ugLane 5: A431 (Human) Cell Lysate at 30 ugLane 6: SH-SY5Y (Human) Cell Lysate at 30 ugPrimary: Anti-UAP56 (SL20587R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 49 kDObserved band size: 49 kD
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Datasheet:


This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2) gene. [provided by RefSeq, Feb 2011]

Function:
Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between ALYREF/THOC4 and the cap-binding protein NCBP1. DDX39B functions as a bridge between ALYREF/THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and ALYREF/THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and ALYREF/THOC4.

Subunit:
Homodimer, and heterodimer with DDX39A. Component of the THO complex, which is composed of THOC1, THOC2, THOC5, THOC6 and THOC7. Together with THOC3, ALYREF/THOC4 and DDX39B, THO forms the transcription/export (TREX) complex. Component of the spliceosome. Interacts directly with U2AF2. Interacts directly with ALYREF/THOC4 and is necessARy for ALYREF/THOC4 recruitment to spliced mRNA. Interacts with RBM8A, RNPS1 and SRRM1. Interacts with FYTTD1/UIF and THOC1. Interacts with human cytomegalovirus/HHSLV5 protein UL69. Interacts with MX1.

Subcellular Location:
Nucleus. Nucleus speckle. Cytoplasm. Note=Can translocate to the cytoplasm in the presence of MX1.

Similarity:
Belongs to the DEAD box helicase family. DECD subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase SLCterminal domain.


SWISS:
Q13838

Gene ID:
7919

Database links:

Entrez Gene: 540191 Cow

Entrez Gene: 7919 Human

Entrez Gene: 53817 Mouse

Entrez Gene: 448813 Pig

Entrez Gene: 114612 Rat

Omim: 142560 Human

SwissProt: Q3T147 Cow

SwissProt: Q13838 Human

SwissProt: Q9Z1N5 Mouse

SwissProt: Q29024 Pig

SwissProt: Q63413 Rat

Unigene: 254042 Human

Unigene: 439827 Mouse

Unigene: 202950 Rat



Picture

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Liver (Rat) Lysate at 40 ug
Lane 5: A431 (Human) Cell Lysate at 30 ug
Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug
Primary: Anti-UAP56 (SL20587R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
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