Rabbit Anti-COL10A1 antibody

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Collagen type X; Col10a 1; Collagen alpha 1(X) chain; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia); Collagen type X alpha 1; Collagen X alpha 1 polypeptide; CollagenX; fa66d11; fb10c08; OTTHUMP00000040411; Procollagen type X alpha 1; Schm

Cat:

SLM52040R

Species Reactivity：

Rat,(predicted: Human,Mouse,)

Immunogen：

KLH conjugated synthetic peptide derived from human COL10A1::501-600/680

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: Liver (Rat) Lysate at 40 ugPrimary: Anti-Collagen X (SLM52040R) at 1/500 dilutionSecondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilutionPredicted band size: 73 kDObserved band size: 66 kDParaformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (SLM54040R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $316.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008].

Function:
Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage.

Subunit:
Homotrimer.

Subcellular Location:
Secreted, extracellular space, extracellular matrix.

Tissue Specificity:
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

DISEASE:
Schmid type metaphyseal chondrodysplasia (SMCD) [MIM:156500]: Dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Contains 1 C1q domain.

SWISS:
A1L4P2

Gene ID:
1300

Database links:

Entrez Gene: 282416 Cow

Entrez Gene: 1300 Human

Entrez Gene: 12813 Mouse

Entrez Gene: 25681 Rat

Omim: 120110 Human

SwissProt: P2646 Cow

SwissProt: A1L4P2 Human

SwissProt: Q03692 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MMP9) Monoclonal Antibody, Unconjugated (SLM54040R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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