Rabbit Anti-Alas1 (1G11) antibody

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mitochondrial; nonspecific; 5 aminolevulinate synthase; 5 aminolevulinate synthase nonspecific mitochondrial; 5 aminolevulinic acid synthase; 5-aminolevulinate synthase; 5-aminolevulinic acid synthase 1; Alas 1; ALAS 3; ALAS; ALAS H; ALAS N; ALAS-H; alaS1

Cat:

SLM52012R

Species Reactivity：

Human,(predicted: Mouse,)

Immunogen：

recombinant human Alas1 C-terminal 200aa

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000IP=1:20-100IHC-P=1:50-200IHC-F=1:50-200Flow-Cyt=1:100-500ICC=1:50-200IF=1:50-200（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Sample: Lane 1: JAR (Human) Cell Lysate at 30 ugLane 2: Huvec (Human) Cell Lysate at 30 ugLane 3: Hela (Human) Cell Lysate at 30 ugLane 4: HepG2 (Human) Cell Lysate at 30 ugLane 5: MCF-7 (Human) Cell Lysate at 30 ugLane 6: K562 (Human) Cell Lysate at 30 ugLane 7: Raji (Human) Cell Lysate at 30 ugPrimary: Anti-Alas1 (SLM52012R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 71/65 kDObserved band size: 65 kD

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Unit:

Price: $316.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals. The erythroid-specific isoenzyme, ALAS-E, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver. ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells. Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H. The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, NF-E2 and EKLF. Since the ALAS gene maps to the X chromosome, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia.

Subunit:
Homodimer.

Subcellular Location:
Mitochondrion matrix

Similarity:
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

SWISS:
P13196

Gene ID:
211

Database links:

Entrez Gene: 211 Human

Entrez Gene: 11655 Mouse

Entrez Gene: 65155 Rat

Omim: 125290 Human

SwissProt: P13196 Human

SwissProt: Q8VC19 Mouse

SwissProt: P13195 Rat

Unigene: 476308 Human

Unigene: 290578 Mouse

Unigene: 97126 Rat

Picture

Sample:
Lane 1: JAR (Human) Cell Lysate at 30 ug
Lane 2: Huvec (Human) Cell Lysate at 30 ug
Lane 3: Hela (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
Lane 6: K562 (Human) Cell Lysate at 30 ug
Lane 7: Raji (Human) Cell Lysate at 30 ug
Primary: Anti-Alas1 (SLM52012R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71/65 kD
Observed band size: 65 kD

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