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Home > Product > Antibody > Rabbit Anti-c-Fos antibody
Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogen
Cat:
SLM52034R
Species Reactivity：
Rat,(predicted: Human,Mouse,)
Immunogen：
Recombinant human c-Fos protein (50-200aa)
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
WB=1:500-1000IP=1:20-100IHC-P=1:50-100IHC-F=1:50-100ICC=1:50-100IF=1:50-100（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Rabbit
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-Fos) Monoclonal Antibody, Unconjugated (SLM52034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Datasheet:


The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in the SLCterminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with phosphorylation on Ser-374 being the major sitefor protein stabilization on NGF stimulation. Phosphorylation onSer-362 and Ser-374 primes further phosphorylations on Thr-325 andThr-331 through promoting docking of MAPK to the DEF domain.Phosphorylation on Thr-232, induced by HA-RAS, activates thetranscriptional activity and antagonizes sumoylation.Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes toosteoblast transformation (By similarity). [PTM] Constitutively sumoylated by SUMO1, SUMO2 and SUMO3.Desumoylated by SENP2. Sumoylation requires heterodimerization withJUN and is enhanced by mitogen stimulation. Sumoylation inhibitsthe AP-1 transcriptional activity and is, itself, inhibited byRas-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

SWISS:
P01100

Gene ID:
2353

Database links:

Entrez Gene: 2353 Human

Entrez Gene: 14281 Mouse

Entrez Gene: 314322 Rat

Omim: 164810 Human

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Unigene: 246513 Mouse

Unigene: 103750 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-Fos) Monoclonal Antibody, Unconjugated (SLM52034R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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