The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008].
Function:
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Subunit:
Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase. Interacts with CCNF; interaction is required for nuclear localization.
Subcellular Location:
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome.
Post-translational modifications:
Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.
Similarity:
Belongs to the cyclin family. Cyclin AB subfamily.
SWISS:
P24860
Gene ID:
891
Database links:
Entrez Gene: 891 Human
Entrez Gene: 268697 Mouse
Entrez Gene: 25203 Rat
Omim: 123836 Human
SwissProt: P14635 Human
SwissProt: P24860 Mouse
SwissProt: P30277 Rat
Unigene: 23960 Human
Unigene: 260114 Mouse
Unigene: 380027 Mouse
Unigene: 482545 Mouse
Unigene: 9232 Rat
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Sample:
Lane 1: Hela cell lysate
Lane 2: Daudi cell lysate
Lane 3: K562 cell lysate
Primary: Anti-Cyclin B1 (SLM52044R) at 1/500 dilution
Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution
Predicted band size: 48 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin B1) Monoclonal Antibody, Unconjugated (SLM52044R) at 1:50 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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