This gene belongs to subfamily P of the forkhead box (FOX) transcription factor family. Forkhead box transcription factors play important roles in the regulation of tissue- and cell type-specific gene transcription during both development and adulthood. Forkhead box P1 protein contains both DNA-binding- and protein-protein binding-domains. This gene may act as a tumor suppressor as it is lost in several tumor types and maps to a chromosomal region (3p14.1) reported to contain a tumor suppressor gene(s). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function:
Transcriptional repressor. It plays an important role in the specification and differentiation of lung epithelium. Can act with CTBP1 to synergistically repress transcription but CTPBP1 is not essential. Essential transcriptional regulator of B cell development.
Subunit:
Forms homodimers and heterodimers with FOXP2 and FOXP4. Dimerization is required for DNA-binding. Interacts with CTBP1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Highest expression in the lung, brain, and spleen. Lower expression in heart, skeletal muscle, kidney, small intestine (isoform 3 not present) and liver.
Similarity:
Contains 1 C2H2-type zinc finger.
Contains 1 fork-head DNA-binding domain.
SWISS:
Q9H334
Gene ID:
27086
Database links:
Entrez Gene: 27086 Human
Entrez Gene: 108655 Mouse
Entrez Gene: 29796 Rat
SwissProt: Q9H334 Human
SwissProt: P58462 Mouse
SwissProt: Q498D1 Rat
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Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:2000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: HeLa, 2: MCF-7, 3: Daudi
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 75kDa
Observed MW: 50-95 kDa
Tissue: rat colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM52078R
Tissue: mouse colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM52078R
Tissue: human colon
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM52078R
Tissue: human colon carcinoma
Section type: Formalin fixed & Paraffin -
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary ab dilution: 1:1000
Primary ab incubation condition: 1 hour at
room temperature
Secondary ab: Anti-Rabbit and Mouse Polymer HRP
Counter stain:
Hematoxylin (Blue)
Comment: Color brown is the positive signal
for SLM52078R
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