Mouse Anti-HP1 alpha antibody

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Antigen p25; HP1-α; CBX5; CBX5_HUMAN; Chromobox homolog 5 (HP1 alpha homolog, Drosophila); Chromobox homolog 5; Chromobox protein homolog 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1 homolog alpha; HP1 alpha; HP

Cat:

SLM33377M

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

Recombinant human HP1 alpha Protein:full length

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:1000-5000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ugPrimary: Anti- HP1 alpha (SLM33377M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDSample: MOLT-4(Human) Cell Lysate at 30 ugSiha(Human) Cell Lysate at 30 ugA549(Human) Cell Lysate at 30 ugPrimary: Anti- HP1 alpha (SLM33377M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kDSample: MCF-7 Cell (Human) Lysate at 40 ugPrimary: Anti-HP1 alpha (SLM33377M) at 1/2 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 24 kDSample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti-HP1 alpha (SLM33377M) at 1/2 000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 24 kDSample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti- HP1 alpha (SLM33377M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 22 kD Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (SLM33377M 7E3) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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HP1 alpha is a component of heterochromatin. It recognizes and binds histone H3 tails methylated at Lys-9, leading to epigenetic repression. HP1 alpha may interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane.
HP1 proteins are relatively small proteins (~25 kDa) with a conserved amino-terminal chromo domain and a structurally related carboxy-terminal motif, the chromo shadow domain. Both domains of HP1 are required for binding to native chromatin in vivo, but they contribute differentially to binding in euchromatin and heterochromatin.

Function:
Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Subunit:
Interacts with SUV420H1 and SUV420H2. Interacts with HP1BP3. Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with BAHD1, MIS12 and DSN1. Interacts with POGZ; POGZ and PXVXL motif-containing proteins such as INCENP and TRIM28 compete for interaction with CBX5. Interacts with INCENP and TRIM24. Interacts with JC virus agnoprotein; this interaction induces the dissociation of CBX5 from LBR, resulting in destabilization of the nuclear envelope. Interacts with CHAMP1. Interacts with ASXL1.

Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.

Post-translational modifications:
Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
Ubiquitinated.

Similarity:
Contains 2 chromo domains.

SWISS:
P45973

Gene ID:
23468

Database links:

Entrez Gene: 538885 Cow

Entrez Gene: 477593 Dog

Entrez Gene: 23468 Human

Entrez Gene: 12419 Mouse

Entrez Gene: 300266 Rat

Omim: 604478 Human

SwissProt: P45973 Human

SwissProt: Q61686 Mouse

Unigene: 349283 Human

Unigene: 262059 Mouse

Picture

Sample:
MOLT-4(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Primary: Anti- HP1 alpha (SLM33377M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD

Sample: MCF-7 Cell (Human) Lysate at 40 ug
Primary: Anti-HP1 alpha (SLM33377M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD

Sample:
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-HP1 alpha (SLM33377M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD

Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- HP1 alpha (SLM33377M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HP1 alpha) Monoclonal Antibody, Unconjugated (SLM33377M 7E3) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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