Mouse Anti-HSP90 alpha antibody

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HSP-90 alpha; HSP 86; HSP 86; Renal carcinoma antigen NY REN 38; Heat shock 86 kDa; Heat shock 90kDa protein 1 alpha; Heat shock protein 90kDa alpha (cytosolic) class A member 1; heat shock protein 90kDa alpha (cytosolic), class A member 2; Heat shock pro

Cat:

SLM33210M

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

Recombinant human HSP90 alpha Protein:549-692/732

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Hela Cell (Human) Lysate at 40 ugNIH/3T3 Cell (Mouse) Lysate at 40 ugPrimary: Anti-HSP90 alpha (SLM33210M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 98 kDSample: 293T(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 40 ugPrimary: Anti-HSP90 alpha (SLM33210M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kDSample: Lane 1: Mouse Testis tissue lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Mouse Placenta tissue lysates Lane 4: Rat Placenta tissue lysates Lane 5: Human U251 cell lysates Lane 6: Human Molt-4 cell lysates Lane 7: Human HL60 cell lysates Lane 8: Human A549 cell lysates Primary: Anti-HSP90 alpha (SLM33210M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 81 kDObserved band size: 81 kDParaformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33210M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33210M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Hsp90 (heat shock protein 90) is a molecular chaperone and is one of the most abundant proteins in unstressed cells. It is an ubiquitous molecular chaperone found in eubacteria and all branches of eukarya, but it is apparently absent in archaea. Whereas cytoplasmic Hsp90 is essential for viability under all conditions in eukaryotes, the bacterial homologue HtpG is dispensable under non-heat stress conditions.

Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Subunit:
Homodimer. Interacts with AHSA1, FNIP1, HSF1, SMYD3 and TOM34. Interacts with TERT; the interaction, together with PTGES3, is required for correct assembly and stabilization of the TERT holoenzyme complex. Interacts with CHORDC1 and DNAJC7. Interacts with STUB1 and UBE2N; may couple the chaperone and ubiquitination systems.

Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity and the activation of eNOS by HSP90AA1.

Similarity:
Belongs to the heat shock protein 90 family.

SWISS:
P07900

Gene ID:
364

Database links:

Entrez Gene: 364 Human

Entrez Gene: 15519 Mouse

Entrez Gene: 299331 Rat

Omim: 140571 Human

SwissProt: P07900 Human

SwissProt: P07901 Mouse

SwissProt: P82995 Rat

Unigene: 525600 Human

Unigene: 700831 Human

Unigene: 1843 Mouse

Unigene: 341186 Mouse

Unigene: 119867 Rat

热休克蛋白-90α主要在类肌细胞、间质细胞、支持细胞核及生精细胞的胞浆表达; HSP-90 alpha与肿瘤的关系密切，在许多肿瘤细胞中HSP90α表达升高. Picture

Sample:
293T(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 40 ug
Primary: Anti-HSP90 alpha (SLM33210M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD

Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Rat Placenta tissue lysates
Lane 5: Human U251 cell lysates
Lane 6: Human Molt-4 cell lysates
Lane 7: Human HL60 cell lysates
Lane 8: Human A549 cell lysates
Primary: Anti-HSP90 alpha (SLM33210M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33210M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33210M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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