Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Mouse Placenta tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Rat Placenta tissue lysates
Lane 6: Human U251 cell lysates
Lane 7: Human Molt-4 cell lysates
Lane 8: Human HL60 cell lysates
Lane 9: Human A549 cell lysates
Primary: Anti-HSP90 alpha (SLM33212M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 85 kD
Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti- HSP90 alpha (SLM33212M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 85 kD
Sample:
Liver (Mouse) Lysate at 40 ug
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-HSP90 alpha (SLM33212M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33212M-9E5) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 alpha) Monoclonal Antibody, Unconjugated (SLM33212M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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