[KO验证抗体] Mouse Anti-HSP90 beta antibody

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HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Hea

Cat:

SLM33136M

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human HSP90 beta

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Hela KO HSP90 beta(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugPrimary: Anti-HSP90 beta (SLM33136M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilutionPredicted band size: 84 kDObserved band size: 84 kDSample: K562 (Human) Lysate at 30 ugPrimary: Anti- HSP90 beta (SLM33136M) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 84 kDObserved band size: 84 kDSample: Hela (Human) Lysate at 30 ugPrimary: Anti- HSP90 beta (SLM33136M) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 84 kDObserved band size: 84 kDSample: Lane 1: A431 (Human) Cell Lysate at 30 ugLane 2: Hela (Human) Cell Lysate at 30 ugLane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ugLane 4: K562 (Human) Cell Lysate at 30 ugLane 5: HepG2 (Human) Cell Lysate at 30 ugLane 6: U2os (Human) Cell Lysate at 30 ugLane 7: A549 (Human) Cell Lysate at 30 ugLane 8: HL60 (Human) Cell Lysate at 30 ugLane 9: Jurkat (Human) Cell Lysate at 30 ugLane 10: Testis (Mouse) Lysate at 40 ugLane 11: Testis (Rat) Lysate at 40 ugLane 12: Cerebrum (Mouse) Lysate at 40 ugLane 13: Cerebrum (Rat) Lysate at 40 ugPrimary: Anti-HSP90 beta (SLM33136M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 90 kDObserved band size: 86 kDParaformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of SLM33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of SLM33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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Unit:

Price: $236.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].

Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.

Subunit:
Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.

Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Post-translational modifications:
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).

Similarity:
Belongs to the heat shock protein 90 family.

SWISS:
P08238

Gene ID:
3326

Database links:

Entrez Gene: 3326 Human

Entrez Gene: 15516 Mouse

Entrez Gene: 301252 Rat

Omim: 140572 Human

SwissProt: P08238 Human

SwissProt: P11499 Mouse

SwissProt: P34058 Rat

Unigene: 509736 Human

Unigene: 236 Mouse

Unigene: 98667 Rat

HSP是细胞受应激原刺激后诱导产生的一组应激蛋白，与肿瘤发生、增殖及分化有关，HSP90β能够抑制细胞凋亡与分化。 Picture

Sample:
K562 (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (SLM33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD

Sample:
Hela (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (SLM33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: A549 (Human) Cell Lysate at 30 ug
Lane 8: HL60 (Human) Cell Lysate at 30 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: Testis (Mouse) Lysate at 40 ug
Lane 11: Testis (Rat) Lysate at 40 ug
Lane 12: Cerebrum (Mouse) Lysate at 40 ug
Lane 13: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-HSP90 beta (SLM33136M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 86 kD

Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of SLM33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of SLM33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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