Mouse Anti-LC3A antibody | Sunlong Medical

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ATG8E; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; LC3; MAP1 light chain 3 like protein 1; MAP1 light chain 3-like protein 1; MAP1A/1B light chain 3 A; MAP1A/MAP1B LC3 A; MAP1A/MAP1B light chain 3 A; MAP1ALC3; MAP1BLC

Cat:

SLM33309M

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

KLH conjugated synthetic peptide derived from human LC3A:1-100/121

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:200-800（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ugLane 2: Cerebrum (Mouse) Lysate at 40 ugLane 3: U251 (Human) Cell Lysate at 30 ugLane 4: SiHa (Human) Cell Lysate at 30 ugLane 5: Cerebellum (Rat) Lysate at 40 ugLane 6: Cerebellum (Mouse) Lysate at 40 ugLane 7: Testis (Rat) Lysate at 40 ugLane 8: Testis (Mouse) Lysate at 40 ugPrimary: Anti-LC3A (SLM33309M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 17 kDObserved band size: 15 kDSample: Hela Cell (Human) Lysate at 40 ugPrimary: Anti- LC3A (SLM33309M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 14/16 kDObserved band size: 16 kDParaformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-FITC) for 90 minutes, and DAPI for nuclei staining.Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved SLCterminal Gly residue; MAP1LC3B does not undergo SLCterminal cleavage and exists in a single modified form.

Function:
Probably involved in formation of autophagosomal vacuoles (autophagosomes).

Subunit:
3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with SQSTM1. Interacts with TP53INP1 and TP53INP2.

Subcellular Location:
Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes.

Tissue Specificity:
Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Post-translational modifications:
The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Similarity:
Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).

SWISS:
Q9H492

Gene ID:
84557

Database links:

Entrez Gene: 84557 Human

Entrez Gene: 66734 Mouse

Entrez Gene: 362245 Rat

Omim: 601242 Human

SwissProt: Q9H492 Human

SwissProt: Q91VR7 Mouse

SwissProt: Q6XVN8 Rat

Unigene: 632273 Human

Unigene: 196239 Mouse

Unigene: 3135 Rat

Picture

Sample:
Hela Cell (Human) Lysate at 40 ug
Primary: Anti- LC3A (SLM33309M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 14/16 kD
Observed band size: 16 kD

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LC3A) Monoclonal Antibody, Unconjugated (SLM33309M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

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