Mouse Anti-AFP(A2) antibody

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Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.

Cat:

SLM1621M

Species Reactivity：

Human,Rat,

Immunogen：

AFP protein:full length

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: AFP recombinded protein lysate at 100ng; Primary: Anti-AFP(A4) (SLM1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kDObserved band size: 65 kDSample: Lane 1: Rat Embryo Lysates Lane 2: Rat Placenta Lysates Primary: Anti-AFP(A2) (SLM1621M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 65kDaObserved band size: 65kDaSample: AFP recombinded protein lysate at 100ng; Primary: Anti-AFP(A4) (SLM1622M) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-mouse IgG(SL0296G-HRP) at 1: 5000 dilution; Predicted band size: 65 kDObserved band size: 65 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (SLM1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.

SWISS:
P02771

Gene ID:
174

Database links:

Entrez Gene: 174 Human

Omim: 104150 Human

SwissProt: P02771 Human

Unigene: 53768 Human

Picture

Sample:
Lane 1: Rat Embryo Lysates
Lane 2: Rat Placenta Lysates
Primary: Anti-AFP(A2) (SLM1621M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65kDa
Observed band size: 65kDa

Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AFP(A2)) Polyclonal Antibody, Unconjugated (SLM1621M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-cy3) for 90 minutes, and DAPI for nuclei staining.

Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 24.94 ng/mL.

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Specific References (10) | SLM1621M has been referenced in 10 publications.

[IF=6.042] Chen Z et al. Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric ELISA.(2018) Anal. Chem. ELISA ; Human.

PubMed:30557502

[IF=5.88] Zhang, Xin, et al. "Spectrum-Based Electrochemiluminescent Immunoassay with Ternary CdZnSe Nanocrystals as Labels." Analytical Chemistry (2016).

PubMed:27266486

[IF=4.52] Zhao, Changrong, et al. "Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition." Analytica Chimica Acta (2014). other ;

PubMed:25261898

[IF=3.998] A. Ikeda. et al. Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep-Uk. 2020 Nov;10(1):1-14 ICC ; Mouse.

PubMed:3643957

[IF=6.277] Zhao CR et al. Highly sensitive and selective electrochemical immunosensors by substrate-enhanced electroless deposition of metal nanoparticles onto three-dimensional Graphene@Ni foams. Science Bulletin.2019. Other ;

PubMed:doi:10.1016/j.scib.2019.07.015

[IF=2.475] Huang Y et al. Ag@Au Core⁻Shell Porous Nanocages with Outstanding SERS Activity for Highly Sensitive SERS Immunoassay. Sensors (Basel). 2019 Mar 31;19(7). pii: E1554. ELISA ;

PubMed:30935114

[IF=5.123] Song X et al. Multiplex immunoassays using surface modification-mediated porous layer open tubular capillary.Anal Chim Acta. 2018 Dec 28;1043:1-10. LIF detection&ELISA ; Human.

PubMed:30392656

[IF=7.48] Zong, Chen, et al. "Multilayer hemin/G-quadruplex wrapped gold nanoparticles as tag for ultrasensitive multiplex immunoassay by chemiluminescence imaging." Biosensors and Bioelectronics 43 (2013): 372-378. ELISA ;

PubMed:23356995

[IF=6.45] Lin, Dajie, et al. "Cascade signal amplification for electrochemical immunosensing by integrating biobarcode probes, surface-initiated enzymatic polymerization and silver nanoparticle deposition." Biosensors and Bioelectronics (2014). other ;

PubMed:2592899

[IF=5.83] Zong, Chen, et al. "Chemiluminescence imaging immunoassay of multiple tumor markers for cancer screening." Analytical Chemistry 84.5 (2012): 2410-2415. other ; Human.

PubMed:2264247