Mouse Anti-ICAM1/CD54 antibody

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BB2; CD54 antigen; CD54; ICAM1; ICAM 1; ICAM-1; Cell surface glycoprotein P3.58; Human rhinovirus receptor; Intercellular adhesion molecule 1; Major group rhinovirus receptor; MALA2; MyD10; P3.58; Surface antigen of activated B cells; ICAM1_HUMAN; MALA-2;

Cat:

SLM10697M

Species Reactivity：

Human,

Immunogen：

Recombinant human ICAM1/CD54:321-420/532

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: A549(Human) Cell Lysate at 30 ugMCF-7(Human) Cell Lysate at 30 ugPrimary: Anti-ICAM1'CD54 (SLM10697M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 90 kDSample: HUVEC(Human) Cell Lysate at 30 ugPrimary: Anti- ICAM1/CD54 (SLM10697M) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 56 kDObserved band size: 100 kDParaformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008].

Function:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Subunit:
Homodimer (Probable). Interacts with human herpesvirus 8 MIR2 protein (Probable). Interacts with MUC1 and promotes cell aggregation in epithelial cells. Interacts with ARHGEF26/SGEF. Binds to coxsackievirus A21 capsid proteins and acts as a receptor for this virus.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Post-translational modifications:
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis.

Similarity:
Belongs to the immunoglobulin superfamily. ICAM family.
ontains 5 Ig-like C2-type (immunoglobulin-like) domains.

SWISS:
P05362

Gene ID:
3383

Database links:

Entrez Gene: 3383 Human

Entrez Gene: 15894 Mouse

Entrez Gene: 25464 Rat

Omim: 147168 Human

Unigene: 643447 Human

SwissProt: P05362 Human

SwissProt: P13597 Mouse

SwissProt: Q00238 Rat

Unigene: 435508 Mouse

Unigene: 12 Rat

Picture

Sample:
HUVEC(Human) Cell Lysate at 30 ug
Primary: Anti- ICAM1/CD54 (SLM10697M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 100 kD

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ICAM1/CD54) Monoclonal Antibody, Unconjugated (SLM10697M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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