Mouse Anti-NSE antibody | Sunlong Medical

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Gamma-enolase; Gamma enolase; Neural enolase; Neuron specific enolase; neurone-specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENO2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Neuron specific enolase; Neuron specific gamma enola

Cat:

SLM33072M

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

Recombinant human NSE protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: A431 Cell (Human) Lysate at 40 ugPrimary: Anti- NSE (SLM33072M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDSample: SH-SY5Y (Human) Cell Lysate at 30 ugU87MG (Human) Cell Lysate at 30 ugPrimary: Anti-NSE (SLM33072M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 48 kDObserved band size: 50 kDParaformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of SLM33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of SLM33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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Unit:

Price: $228.00

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Other Information
Citations
Protein Attributes
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This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008].

Function:
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

Subunit:
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular Location:
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Tissue Specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Similarity:
Belongs to the enolase family.

SWISS:
P09104

Gene ID:
2026

Database links:

Entrez Gene: 2026 Human

Entrez Gene: 2767 Mouse

Entrez Gene: 24334 Rat

Omim: 13172 Human

SwissProt: P09104 Human

SwissProt: P17183 Mouse

SwissProt: P07323 Rat

Unigene: 511915 Human

神经元特异性烯醇化酶（NSE）存在于神经元细胞和神经内分泌组织中，起源于神经内分泌细胞的肿瘤可产生过量的NSE。NSE也是小细胞肺癌的检测指标，70％左右的小细胞肺癌患者血中NSE升高，而其他组织型肺癌NSE升高的患者仅为10％～20％。 Picture

Sample:
SH-SY5Y (Human) Cell Lysate at 30 ug
U87MG (Human) Cell Lysate at 30 ug
Primary: Anti-NSE (SLM33072M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of SLM33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NSE) Monoclonal Antibody, Unconjugated (ascites of SLM33072M 15E2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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