The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a SLCterminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
Function:
May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.
Subunit:
Interacts with MAP4K2 and SYTL4. Interacts with SGSM1 and SGSM3 (By similarity). Interacts with OPTN. Interacts with RAB3IP. Interacts with MYO5B. Interacts with PIFO. Interacts with BIRC6/bruce. Interacts with OCRL.
Subcellular Location:
Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Golgi apparatus. Cytoplasm, perinuclear region. Cell projection. Note=Colocalizes with OPTN at the Golgi complex and in vesicular structures close to the plasma membrane. In the GDP-bound form, present in the perinuclear region. Shows a polarized distribution to distal regions of cell protrusions in the GTP-bound form. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB11A in apical membrane initiation sites (AMIS) during the generation of apical surface and luminogenesis.
Similarity:
Belongs to the small GTPase superfamily. Rab family.
SWISS:
P61006
Gene ID:
4218
Database links:
Entrez Gene: 100125881 Cow
Entrez Gene: 403773 Dog
Entrez Gene: 4218 Human
Entrez Gene: 17274 Mouse
Entrez Gene: 117103 Rat
Omim: 165040 Human
SwissProt: Q5F470 Chicken
SwissProt: A4FV54 Cow
SwissProt: P61007 Dog
SwissProt: P61006 Human
SwissProt: P55258 Mouse
SwissProt: P35280 Rat
Unigene: 642874 Human
Unigene: 709846 Human
Unigene: 162811 Mouse
Unigene: 16064 Rat
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Sample:
Raji(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- RAB8A (SLM51077M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 25 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti- RAB8A (SLM51077M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti-RAB8A (SLM51077M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
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