Mouse Anti-CRP(C11F2) antibody

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C-reactive protein; C Reactive Protein; C reactive protein pentraxin related; CRP; MGC88244; PTX 1; PTX1; C-reactive protein(1-205); CRP_HUMAN; Pentraxin 1.

Cat:

SLM0391M

Species Reactivity：

Human,

Immunogen：

Recombinded CRP protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: U2os (Human) Cell Lysate at 30 ugLane 2: Siha (Human) Cell Lysate at 30 ugPrimary: Anti- CRP (SLM0391M) at 1/1000 dilutionAnti- beta-Actin (SL0061R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionIRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 26 kDSample: C Reactive Protein(CRP) Lysate at 40 ugPrimary: Anti- CRP(C11F2) (SLM0391M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 27 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of SLM0391M C11F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of SLM0391M C11F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Polyclonal Antibody, Unconjugated (SLM0391M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Function:
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Subunit:
Homopentamer. Pentaxin (or pentraxin) have a discoid arrangement of 5 non-covalently bound subunits.

Subcellular Location:
Secreted.

Tissue Specificity:
Found in plasma.

Similarity:
Belongs to the pentaxin family.
Contains 1 pentaxin domain.

SWISS:
P02741

Gene ID:
1401

Database links:

Entrez Gene: 1401 Human

Omim: 123260 Human

SwissProt: P02741 Human

Unigene: 76452 Human

SLC反应蛋白-CRP（SLCreactive protin）是与C性肺炎球菌的多糖起反应的一种蛋白，在某些细菌的感染过程中出现在血浆中。 Picture

Sample:
C Reactive Protein(CRP) Lysate at 40 ug
Primary: Anti- CRP(C11F2) (SLM0391M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27 kD

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Monoclonal Antibody, Unconjugated (ascites of SLM0391M C11F2) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CRP(C11F2)) Polyclonal Antibody, Unconjugated (SLM0391M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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