Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Pancreas (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Hela (Human) Cell Lysate at 30 ug
Lane 5: MCF-7 (Human) Cell Lysate at 30 ug
Lane 6: A431 (Human) Cell Lysate at 30 ug
Lane 7: U2os (Human) Cell Lysate at 30 ug
Primary: Anti-GSK-3 Beta (SLM33294M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 45 kD
Sample:
Ovary (Mouse) Lysate at 40 ug
Primary: Anti- GSK-3 Beta (SLM33294M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (SLM33294M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GSK-3 Beta) Monoclonal Antibody, Unconjugated (SLM33294M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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