Mouse Anti-Caspase 8 antibody

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Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CA

Cat:

SLM33190M

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

Recombinant human Caspase 8 Protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:2000-5000ELISA=1:5000-10000IHC-P=1:400-1000IHC-F=1:500-1000IF=1:500-1000（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Hela(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-Caspase 8 (SLM33190M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41'43'55 kD Observed band size: 43 kD Sample: Stomach(Mouse) Lysate at 40 ugSpleen(Mouse) Lysate at 40 ugLymph node(Mouse) Lysate at 40 ugUrinary bladder(Mouse) Lysate at 40 ugPrimary: Anti-Caspase 8 (SLM33190M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 41'43'55 kDObserved band size: 55'53DSample: 293T-UV(Human) Cell Lysate at 30 ugU251-UV(Human) Cell Lysate at 30 ugPrimary: Anti-Caspase 8 (SLM33190M) at 1/800 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41’43’55 kDObserved band size: 43 kDParaformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of SLM33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (SLM33190M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Function:
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Subunit:
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

DISEASE:
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.

Similarity:
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.

SWISS:
Q14790

Gene ID:
841

Database links:

Entrez Gene: 841 Human

Entrez Gene: 12370 Mouse

Entrez Gene: 54474 Rat

Entrez Gene: 12844 Rat

Omim: 601763 Human

SwissProt: Q14790 Human

SwissProt: O89110 Mouse

SwissProt: Q9JHX4 Rat

Unigene: 599762 Human

Unigene: 655983 Human

Unigene: 336851 Mouse

半胱氨酸蛋白酶-8，在凋亡执行阶段起中心作用。 Picture

Sample:
Stomach(Mouse) Lysate at 40 ug
Spleen(Mouse) Lysate at 40 ug
Lymph node(Mouse) Lysate at 40 ug
Urinary bladder(Mouse) Lysate at 40 ug
Primary: Anti-Caspase 8 (SLM33190M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41'43'55 kD
Observed band size: 55'53D

Sample:
293T-UV(Human) Cell Lysate at 30 ug
U251-UV(Human) Cell Lysate at 30 ug
Primary: Anti-Caspase 8 (SLM33190M) at 1/800 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 41’43’55 kD
Observed band size: 43 kD

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (ascites of SLM33190M 4G12) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Monoclonal Antibody, Unconjugated (SLM33190M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

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