Sample:
U87MG(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
HepG2(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
U2os(Human) Cell Lysate at 30 ug
Primary: Anti-GRP78/Bip (SLM33224M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 78 kD
Observed band size: 78 kD
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, SLC0005) at 37°C for 20 min; Antibody incubation with (GRP78/Bip) monoclonol Antibody, Unconjugated (SLM33224M) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.