Sample:
Lung (Mouse) Lysate at 40 ug
Hela(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Uterus (Mouse) Lysate at 40 ug
Primary: Anti- alpha Tubulin (SLM33039M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 53 kD
Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human Jurkat cell Lysates
Lane 3: Human SH-SY5Y cell Lysates
Lane 4: Human U-2 OS cell Lysates
Lane 5: Human A431 cell Lysates
Lane 6: Human A549 cell Lysates
Primary: Anti-Alpha Tubulin (SLM33039M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50kDa
Observed band size: 50kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of SLM33039M 10F11 ) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.