Janus kinase 1 (JAK1) is a member of a new class of non-receptor protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
Function:
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Subunit:
Interacts with FER (By similarity). Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB.
Subcellular Location:
Endomembrane system; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.
Tissue Specificity:
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 2 protein kinase domains.
Contains 1 SH2 domain.
SWISS:
P23458
Gene ID:
3716
Database links:
Entrez Gene: 395681 Chicken
Entrez Gene: 3716 Human
Entrez Gene: 16451 Mouse
Entrez Gene: 397202 Pig
Entrez Gene: 84598 Rat
Omim: 147795 Human
SwissProt: P23458 Human
SwissProt: P52332 Mouse
Unigene: 207538 Human
Unigene: 289657 Mouse
Unigene: 90191 Rat
激酶和磷酸酶（Kinases and Phosphatases）
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Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JAK1) Monoclonal Antibody, Unconjugated (SLM33268M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (JAK1) Monoclonal Antibody, Unconjugated (SLM33268M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Blank control（black line）:MCF-7.
Primary Antibody (green line): Mouse Anti-JAK1 antibody (SLM33268M)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-Mouse IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Mouse IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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