Mouse Anti-Epsilon Tubulin antibody

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dJ142L7.2; Epsilon-tubulin; FLJ22589; FLJ44203; OTTHUMP00000040425; TBE_HUMAN; TUBE 1; TUBE; TUBE1; Tubulin epsilon 1; Tubulin epsilon; Tubulin epsilon chain.

Cat:

SLM33314M

Species Reactivity：

Human,Mouse,(predicted: Rat,)

Immunogen：

KLH conjugated synthetic peptide derived from human Epsilon Tubulin

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: K562(Human) Cell Lysate at 30 ugHL60(Human) Cell Lysate at 30 ugU87MG(Human) Cell Lysate at 30 ugPrimary: Anti- Epsilon Tubulin (SLM33314M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 53 kDObserved band size: 53 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBE) Polyclonal Antibody, Unconjugated (SLM33314M-4C3) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the tubulin superfamily. This protein localizes to the centriolar sub-distal appendages that are associated with the older of the two centrioles after centrosome duplication. This protein plays a central role in organization of the microtubules during centriole duplication. A pseudogene of this gene is found on chromosome 5.[provided by RefSeq, Jan 2009]

Subcellular Location:
Cytoplasm; cytoskeleton; centrosome. Associated with pericentriolar material.

Similarity:
Belongs to the tubulin family.

SWISS:
Q9UJT0

Gene ID:
51175

Database links:

Entrez Gene: 51175 Human

Omim: 607345 Human

SwissProt: Q9UJT0 Human

Unigene: 34851 Human

Picture

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBE) Polyclonal Antibody, Unconjugated (SLM33314M-4C3) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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