Mouse Anti-HAO1 antibody | Sunlong Medical

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Glycolate oxidase; GOX; GOX1; HAO1; HAOX1; HAOX1_HUMAN; Hydroxyacid oxidase 1; MGC142225; MGC142227; OTTHUMP00000030231; (S) 2 hydroxy acid oxidase.

Cat:

SLM33191M

Species Reactivity：

Mouse,Rat,

Immunogen：

Recombinant mouse HAO1 Protein:full length

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Liver(Rat) Lysate at 40 ugPrimary: Anti- HAO1 (SLM33191M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 41 kDObserved band size: 42 kDParaformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HAO1) Monoclonal Antibody, Unconjugated (ascites of SLM33191M Mix) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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GOX is a 370 amino acid protein that is expressed in liver and pancreas. HAO1 is localized to peroxisomes and aids in organic acid metabolism via 2-hydroxyacid oxidase activity. 2-hydroxyacid oxidases, such as HAO1, are enzymes that require a flavin cofactor to oxidize 2-hydroxyacids to 2-ketoacids while reducing oxygen to hydrogen peroxide. HAO1 prefenentially oxidizes the substrate glycolate and also oxidizes other substrates, including 2-hydroxy fatty acids as well as L-?hydroxy acids of moderately short chain lengths. The oxidation of glycolate yields glyoxylate which is utilized for peroxisomal synthesis of glycine. HAO1 is also able to convert glyoxylate to oxalate. HAO1 is thought to play a role in the pathophysiology of hyperoxaluria type 1, which is caused by defects in AGXT, a peroxisomal enzyme, leading to accumulation of glyoxylate. Hyperoxaluria type 1 is characterized by an accumulation of oxalate that is thought to lead to precipitates of calcium oxalate in kidneys which can be fatal.

Function:
Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.

Subcellular Location:
Peroxisome.

Tissue Specificity:
Liver.

Similarity:
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Contains 1 FMN hydroxy acid dehydrogenase domain.

SWISS:
Q9WU19

Gene ID:
15112

Database links:

Entrez Gene: 54363 Human

Entrez Gene: 15112 Mouse

Omim: 605023 Human

SwissProt: Q9UJM8 Human

SwissProt: Q9WU19 Mouse

Unigene: 193128 Human

Unigene: 26634 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HAO1) Monoclonal Antibody, Unconjugated (ascites of SLM33191M Mix) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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