Mouse Anti-AFP(A4) antibody

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Alpha Fetoprotein; Alpha-fetoprotein; alpha 1 fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP; FETA_HUMAN.

Cat:

SLM1622M

Species Reactivity：

Human,

Immunogen：

AFP protein:full length

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Jurkat(Human) Cell Lysate at 30 ugPrimary: Anti-AFP(A4) (SLM1622M) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 65 kDObserved band size: 65 kDMeasured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/mL.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Function:
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit:
Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:
Secreted.

Tissue Specificity:
Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains.

SWISS:
P02771

Gene ID:
174

Database links:

Entrez Gene: 174 Human

Omim: 104150 Human

SwissProt: P02771 Human

Unigene: 53768 Human

Picture

Measured by its binding ability in a indirect ELISA. Immobilized Human AFP protein, His Tag (Cat. SL43140P) at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human AFP Antibody, the EC50 is 20.04 ng/mL.

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