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Home > Product > Antibody > Mouse Anti-CEA(B5) antibody
Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.
Cat:
SLM1624M
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human CEA/CD66e/CEACAM5:full length
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA(B5)) Polyclonal Antibody, Unconjugated (SLM1624M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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Unit:
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Product PDFs
Datasheet:


CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.

Function:
Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.

Subunit:
Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor

Tissue Specificity:
Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Post-translational modifications:
Complex immunoreactive glycoprotein with a MW of 36 kDa comprising 60% carbohydrate.

Similarity:
Belongs to the immunoglobulin superfamily. CEA family.
Contains 7 Ig-like (immunoglobulin-like) domains.

SWISS:
P06731

Gene ID:
1048

Database links:

Entrez Gene: 1048 Human

Omim: 114890 Human

SwissProt: P06731 Human

Unigene: 709196 Human




CEA是一种胚胎性抗原，主要存在于胎儿消化道上皮组织，胰腺和肝癌中。CEA广泛存在各种上皮性肿瘤，尤其是胃肠道恶性肿瘤，CEA分布阳性类型与肿瘤的恶性度有关。CEA与EMA一样可作为上皮性恶性肿瘤的重要标记。
分子量为150-200 kDa。
该抗体与SLM1623M（检测）（Mouse Anti-Human CEA Monoclonal）配对使用；主要用于ELISA试剂盒，胶体金试剂条制备生产都很好。大量订购可来电商议。 Picture

Paraformaldehyde-fixed, paraffin embedded (liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEA(B5)) Polyclonal Antibody, Unconjugated (SLM1624M) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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