Home>Product>Antibody> Mouse Anti-Myc tag antibody
MYC_HUMAN;
avian myelocytomatosis viral oncogene homolog;
bHLHe39;
c-Myc;
class E basic helix-loop-helix protein 39;
MRTL;
MYC;
Myc Epitope Tag;
myc proto-oncogene protein;
myc-related translation/localization regulatory factor;
oncogene c-Myc;
Cat:
SLM33024M
Species Reactivity:
Recombinant protein
Immunogen:
KLH conjugated C-EQKLISEEDL
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Monoclonal
Isotype:
IgG1
Applications:
WB=1:100-5000ELISA=1:5000-10000IP=1:20-100ICC=1:100-500IF=1:100-500not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Mouse
Product Overview:
Sample: Lane 1: Myc-tagged fusion protein Primary: Anti-Myc tag (SLM33024M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: kDObserved band size: 46 kD
Epitope tags are useful for the labeling and detection of proteins using
immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast,insect and mammalian cell systems.
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and SLCJun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
Sample:
Lane 1: Myc-tagged fusion protein
Primary: Anti-Myc tag (SLM33024M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: kD
Observed band size: 46 kD