Mouse Anti-Peroxiredoxin 1 antibody

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Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; PRDX1_HUMAN; Hbp23; Heme binding 23 kDa protein; Macrophage 23 Kd stress protein; Macrophage 23kDa stress protein; Macrophase stress protein 22kDa; Macrophase stress protein 23 kd; MSP23; Natural killer cel

Cat:

SLM33118M

Species Reactivity：

Human,Mouse,Rat,

Immunogen：

Recombinant human Peroxiredoxin 1 Protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: Mouse Spleen tissue lysates Lane 2: Mouse Liver tissue lysates Lane 3: Mouse Cerebrum tissue lysates Lane 4: Rat Spleen tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Human Jurkat cell lysates Lane 7: Human Raji cell lysates Lane 8: Human MCF-7 cell lysates Lane 9: Human A431 cell lysates Lane 10: Human Hela cell lysates Primary: Anti-Peroxiredoxin 1 (SLM33118M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 23 kD Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 1) Monoclonal Antibody, Unconjugated (ascites of SLM33118M 10F9) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jan 2011].

Function:
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.

Subunit:
Homodimer; disulfide-linked, upon oxidation. May form heterodimers with AOP2. Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity).

Subcellular Location:
Cytoplasm. Melanosome.

Post-translational modifications:
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity.

Similarity:
Belongs to the AhpC/TSA family.
Contains 1 thioredoxin domain.

SWISS:
Q06830

Gene ID:
5052

Database links:

Entrez Gene: 281997 Cow

Entrez Gene: 5052 Human

Entrez Gene: 18477 Mouse

Entrez Gene: 117254 Rat

Omim: 176763 Human

SwissProt: Q9JKY1 Chinese Hamster

SwissProt: Q06830 Human

SwissProt: P35700 Mouse

SwissProt: Q63716 Rat

Unigene: 36909 Human

Unigene: 30929 Mouse

Unigene: 2845 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Peroxiredoxin 1) Monoclonal Antibody, Unconjugated (ascites of SLM33118M 10F9) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.

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