Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Function:
Produces green light with a wavelength of 562 nm.
Subcellular Location:
Peroxisome.
Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.
SWISS:
P08659
Gene ID:
N/A
Database links:
SwissProt: P08659 Firefly (Photinus pyralis)
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Sample: Fruit Fly Lysate at 40 ug
Primary: Anti-Firefly Luciferase (SLM33318M) at 1/2 000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61 kD
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