Sample: Jurkat Cell (Human) Lysate at 40 ug
Primary: Anti-NFKB p65 (SLM33059M) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 65 kD
Sample:
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
Primary: Anti-NFKB p65 (SLM33059M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Molt-4(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Raw264.7(Mouse) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-NFKB p65 (SLM33059M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
Tissue/cell:MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,SLC0005) at 37°C for 20 min; Antibody incubation with (NFKB p65) monoclonal Antibody, Unconjugated (SLM33059M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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