Rabbit IgG Fc; IgG Heavy Chain; Mouse anti-Rabbit IgG Fc.
Cat:
SLM33179M
Species Reactivity：
Rabbit,
Immunogen：
rabbit IgG
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
WB=1:500-1000IHC-P=1:100-500IHC-F=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Sample: Rabbit IgG Lysate at 30 ugPrimary: Anti-Rabbit IgG(Fc specific,Heavy Chain) (SLM33179M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 55 kD
IgG antibodies are large molecules of about 150 kDa made of four peptide chains. It contains two identical class γ heavy chains of about 50 kDa and two identical light chains of about 25 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6-linked sialic acid residues.
SWISS: N/A
Gene ID: N/A
Picture
Sample:
Rabbit IgG Lysate at 30 ug
Primary: Anti-Rabbit IgG(Fc specific,Heavy Chain) (SLM33179M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
