Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Clonality:
Monoclonal
Isotype:
IgG1
Applications:
Flow-Cyt=20ul/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Mouse
Product Overview:
Flow cytometry staining of normal human peripheral blood cells with CD34/FITC (SLM30079M-FITC)(filled histogram) or Mouse IgG1 Isotype Control (FITC Conjugate)(C03-11001)(open histogram). Total cells were used for analysis.
The highly glycosylated 75-120 kD antigen CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C, suggesting a putative role in signal transduction. Two isoforms of CD34 have been reported to be generated by alternative splicing. CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.
Function: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Subcellular Location: Membrane; Single-pass type I membrane protein.
Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
Post-translational modifications: Highly glycosylated.
Phosphorylated on serine residues by PKC.
Flow cytometry staining of normal human peripheral blood cells with CD34/FITC (SLM30079M-FITC)(filled histogram) or Mouse IgG1 Isotype Control (FITC Conjugate)(C03-11001)(open histogram). Total cells were used for analysis.