Mouse Anti-human CD33-APC antibody

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CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-

Cat:

SLM30078M-APC

Species Reactivity：

Human,

Format：

Liquid

Storage instructions：

Store at 2-8℃ for one year. Avoid repeated freeze/thaw cycles.

Clonality：

Monoclonal

Isotype：

IgG2a

Applications：

Flow-Cyt=5ul/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Flow cytometry staining of normal human peripheral blood cells with CD33/APC(SLM30078M-APC)(Red histogram) or Mouse IgG2a Isotype Control (APC Conjugate)(C03-2404)(Blue histogram) . Total cells were used for analysis.Blank control:U-937. Primary Antibody (green line): Rabbit Anti-CD33 antibody (SLM30078M-APC) Dilution: 1ug/Test; ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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Unit:

Price: $376.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Function:
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Subunit:
Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Monocytic/myeloid lineage cells.

Post-translational modifications:
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11.
Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like SLVtype (immunoglobulin-like) domain.

SWISS:
P20138

Gene ID:
945

Database links:

Entrez Gene: 945 Human

Omim: 159590 Human

SwissProt: P20138 Human

Unigene: 83731 Human

Picture

Blank control:U-937.
Primary Antibody (green line): Rabbit Anti-CD33 antibody (SLM30078M-APC)
Dilution: 1ug/Test;
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.

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