Mouse Anti-beta 2 Microglobulin (7B5) antibody

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B2M; Beta-2-MG; Beta 2 microglobulin precursor; Beta chain of mhc class 1 proteins; Hdcma22p; B2MG_HUMAN; Beta 2 microglobin; Beta-2 microglobulin precursor; Beta chain of mhc class 1 proteins; Beta chain of MHC class I molecules; Beta-2-microglobulin for

Cat:

SLM41168M

Species Reactivity：

Human,

Immunogen：

Recombinant human beta 2 Microglobulin protein

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG1

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: Jurkat (Human) Cell Lysate at 30 ugLane 2: Hela (Human) Cell Lysate at 30 ugLane 3: HepG2 (Human) Cell Lysate at 30 ugLane 4: Panc-1 (Human) Cell Lysate at 30 ugLane 5: U937 (Human) Cell Lysate at 30 ugPrimary: Anti-beta 2 Microglobulin (SLM41168M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 11 kDObserved band size: 11 kDParaformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta 2 Microglobulin (7B5)) Monoclonal Antibody, Unconjugated (SLM41168M 7B5) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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SLM41168M(capture) is Matched Pair with SLM41182M(detection).
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Sep 2009].

Function:
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Subunit:
Heterodimer of an alpha chain and a beta chain. Beta-2-microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis.

Subcellular Location:
Secreted. Note=Detected in serum and urine.

Post-translational modifications:
Glycation of Ile-21 is observed in long-term hemodialysis patients.

DISEASE:
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

Similarity:
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

SWISS:
P61769

Gene ID:
567

Database links:

Entrez Gene: 567 Human

Entrez Gene: 12010 Mouse

Entrez Gene: 24223 Rat

Omim: 109700 Human

SwissProt: P61769 Human

SwissProt: P01887 Mouse

SwissProt: P07151 Rat

Unigene: 534255 Human

Unigene: 702947 Human

Unigene: 163 Mouse

Unigene: 1868 Rat

Picture

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta 2 Microglobulin (7B5)) Monoclonal Antibody, Unconjugated (SLM41168M 7B5) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

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