Home>Product>Antibody> Mouse Anti-Topoisomerase I antibody
DNA topoisomerase 1; DNA topoisomerase I; NUP98 fusion gene; TOP 1; TOP I; TOP1; TOP1_HUMAN; TOPI; Topoisomerase 1; Topoisomerase1; TopoisomeraseI; Type I DNA topoisomerase.
Cat:
SLM51396M
Species Reactivity：
Human,(predicted: Mouse,Rat,)
Immunogen：
Recombinant human Topoisomerase I
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG1
Applications：
WB=1:500-2000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Sample: MOLT-4 (Human) Cell Lysate at 30 ugMCF-7 (Human) Cell Lysate at 30 ugSiHa (Human) Cell Lysate at 30 ugK562 (Human) Cell Lysate at 30 ugPrimary: Anti-Topoisomerase I (SLM51396M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 91 kDObserved band size: 91 kD
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
Function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Subcellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
Post-translational modifications: Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
DISEASE: Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
Similarity: Belongs to the eukaryotic type I topoisomerase family.
Sample:
MOLT-4 (Human) Cell Lysate at 30 ug
MCF-7 (Human) Cell Lysate at 30 ug
SiHa (Human) Cell Lysate at 30 ug
K562 (Human) Cell Lysate at 30 ug
Primary: Anti-Topoisomerase I (SLM51396M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 91 kD
Observed band size: 91 kD
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Specific References (1) | SLM51396M has been referenced in 1 publications.
[IF=3.407] Xu W et al. Tetrandrine and cepharanthine induce apoptosis through caspase cascade regulation, cell cyclearrest, MAPK activation and PI3K/Akt/mTOR signal modification in glucocorticoid resistant human leukemia Jurkat T cells. Chem Biol Interact. 2019 Jun 28;310:108726. WB ; Human.
