Mouse Anti-CK18 antibody | Sunlong Medical

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K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;

Cat:

SH0006

Species Reactivity：

Human,

Immunogen：

KLH conjugated synthetic peptide derived from human CK18

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Clonality：

Monoclonal

Isotype：

IgG

Applications：

IHC-P=1:200-800IHC-F=1:200-800（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK18) Polyclonal Antibody, Unconjugated (SH0006) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Function:
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Post-translational modifications:
Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P05783

Gene ID:
3875

Database links:

Entrez Gene: 3875 Human

Omim: 29670 Human

SwissProt: P05783 Human

Unigene: 406013 Human

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