platelet glycoprotein IIb of IIb/IIIa complex; Integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); GPIIb; GTA; HPA3; CD 41; CD41 antigen; CD41a; CD41b; GP2b; GPalpha IIb; GPalphaIIb; Integrin alpha IIb; Integrin alpha IIb pre
Cat:
SLM33112M
Species Reactivity：
Human,
Immunogen：
KLH conjugated synthetic peptide derived from human ITGA2B
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
WB=1:500-2000ELISA=1:5000-10000Flow-Cyt=1ug/Testnot yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Sample: Lane 1: HL60 (Human) Cell Lysate at 30 ugLane 2: Raji (Human) Cell Lysate at 30 ugPrimary: Anti-CD41/Integrin alpha 2b (SLM33112M) at 1/1000 dilutionAnti-beta-Actin (SL0061R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 125 kDObserved band size: 125 kD
This gene encodes a member of the integrin alpha chain family of proteins. The encoded preproprotein is proteolytically processed to generate light and heavy chains that associate through disulfide linkages to form a subunit of the alpha-IIb/beta-3 integrin cell adhesion receptor. This receptor plays a crucial role in the blood coagulation system, by mediating platelet aggregation. Mutations in this gene are associated with platelet-type bleeding disorders, which are characterized by a failure of platelet aggregation, including Glanzmann thrombasthenia. [provided by RefSeq, Jan 2016]
Function: Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells.
Subcellular Location: Membrane.
DISEASE: Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
Similarity: Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Sample:
Lane 1: HL60 (Human) Cell Lysate at 30 ug
Lane 2: Raji (Human) Cell Lysate at 30 ug
Primary:
Anti-CD41/Integrin alpha 2b (SLM33112M) at 1/1000 dilution
Anti-beta-Actin (SL0061R) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 125 kD
