Mouse Anti-alpha Tubulin (Acetyl Lys40) antibody

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Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; TBA1A_HUMAN; TBA1B_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alph

Cat:

SLM33235M

Species Reactivity：

Human,Mouse,Zebrafish,(predicted: Rat,)

Immunogen：

KLH conjugated synthesised acetylpeptide derived from human alpha Tubulin around the acetylation site of Acetyl Lys40:SD(Ac-K)TI

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:500-1000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ugLane 2: LOVO (Human) Cell Lysate at 30 ugLane 3: A431 (Human) Cell Lysate at 30 ugLane 4: Hela (Human) Cell Lysate at 30 ugPrimary: Anti-alpha Tubulin (Acetyl Lys40) (SLM33235M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 52 kDSample: HepG2(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugNIH/3T3(Mouse) Cell Lysate at 30 ugA431(Human) Cell Lysate at 30 ugPrimary: Anti-alpha Tubulin (Acetyl Lys40) (SLM33235M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilutionPredicted band size: 52 kDObserved band size: 53 kD

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Function:
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Subunit:
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Subcellular Location:
Cytoplasm, cytoskeleton.

Tissue Specificity:
Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex

Post-translational modifications:
Some glutamate residues at the SLCterminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).

Similarity:
Belongs to the tubulin family.

SWISS:
P68366

Gene ID:
7277

Database links:

Entrez Gene: 7277 Human

Entrez Gene: 22145 Mouse

Entrez Gene: 316531 Rat

SwissProt: P68366 Human

SwissProt: P68368 Mouse

SwissProt: Q5XIF6 Rat

Picture

Sample:
HepG2(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
NIH/3T3(Mouse) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Primary: Anti-alpha Tubulin (Acetyl Lys40) (SLM33235M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 53 kD

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