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Home > Product > Antibody > Mouse Anti-Claudin 2 antibody
Claudin-2; Claudin2; CLD2_HUMAN; CLDN 2; CLDN2; OTTHUMP00000023793; SP 82; SP82.
Cat:
SLM33414M
Species Reactivity：
Human,(predicted: Mouse,Rat,)
Immunogen：
KLH conjugated synthetic peptide derived from human Claudin 2:130-230/230aa
Format：
Liquid
Storage instructions：
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration：
1mg/ml
Clonality：
Monoclonal
Isotype：
IgG
Applications：
IHC-P=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host：
Mouse
Product Overview：
Paraformaldehyde-fixed, paraffin embedded (Human gallbladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 2) Monoclonal Antibody, Unconjugated (SLM33414M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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Product PDFs
Datasheet:


The claudin superfamily consists of structurally related proteins that are important structural and functional components of tight junctions. Claudin-2, also known as CLDN2 or SP82, is a 230 amino acid multi-pass membrane protein that localizes to the cell junctions and belongs to the claudin superfamily. Able to form homopolymers of heteropolymers with other claudin family members, claudin-2 plays an essential role in mediating calcium-independent cell-adhesion activity that is necessary for tight junction-specific obliteration of the intercellular space. Overexpression of claudin-2 is associated with a variety of diseases, including lung cancer, colorectal cancer, gastrointestinal carcinomas and inflammatory bowel disease, further affirming the importance of claudin-2 in cell adhesion. The gene encoding claudin-2 maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes.

Function:
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Subunit:
Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity).

Subcellular Location:
Cell junction; tight junction. Cell membrane.

Similarity:
Belongs to the claudin family.

SWISS:
P57739

Gene ID:
9075

Database links:

Entrez Gene: 404089 Cow

Entrez Gene: 9075 Human

Entrez Gene: 12738 Mouse

Entrez Gene: 300920 Rat

Omim: 300520 Human

SwissProt: P57739 Human

SwissProt: O88552 Mouse

Unigene: 522746 Human

Unigene: 117068 Mouse

Unigene: 93431 Rat



Picture

Paraformaldehyde-fixed, paraffin embedded (Human gallbladder); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Claudin 2) Monoclonal Antibody, Unconjugated (SLM33414M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
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