Sample:
Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33184P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- AmCyan-Tag (SLM33184M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD