Home > Product > Antibody > Mouse Anti-AmCyan antibody
AmCyan Tag; AmCyan-Tag; 野生型天竺葵青色荧光蛋白; 蓝色荧光蛋白AmCyan标签; 蓝色荧光蛋白标签;
Cat:
SLM33184M
Species Reactivity:
Recombinant protein
Immunogen:
Recombinant AmCyan
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Monoclonal
Isotype:
IgG
Applications:
WB=1:1000-5000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Mouse
Product Overview:
Sample: Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33184P) at 4 ugLane 2: Control (-) E.coli Lysate at 4 ugPrimary: Anti- AmCyan-Tag (SLM33184M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 25 kDObserved band size: 25 kD
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Unit:
Price: $
Product PDFs
Datasheet:


AmCyan1 is a human codon-optimized variant of the wild-type Anemonia majano cyan fluorescent protein (AmCyan) that exhibits enhanced emission characteristics (excitation and emission maxima: 458nm and 489nm, respectively). DD-AmCyan1 is a modified version of AmCyan1 that is tagged on its N-terminus with the ProteoTuner destabilization domain (DD). The presence of this destabilization domain causes rapid, proteasomal degradation of the fluorescent fusion protein; however, when the membrane permeant ligand Shield1 is added to the medium, it binds to the destabilization domain and protects the fusion protein from degradation.

SWISS:
N/A

Gene ID:
N/A

Picture

Sample:
Lane 1: AmCyan-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33184P) at 4 ug
Lane 2: Control (-) E.coli Lysate at 4 ug
Primary: Anti- AmCyan-Tag (SLM33184M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 25 kD
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