Mouse Anti-TAP-Tag antibody

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TAP Tag; TAPTag; Tandem affinity purification

Cat:

SLM33133M

Species Reactivity：

Recombinant protein

Immunogen：

Recombinant TAP-Tag

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug Primary: Anti-TAP-Tag (SLM33133M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 51 kDObserved band size: 51 kD

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Unit:

Price: $196.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the SLCterminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.

SWISS:
N/A

Gene ID:
N/A

Picture

Sample:
Lane 1: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug
Lane 2: TAP-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug
Primary: Anti-TAP-Tag (SLM33133M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD

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