Mouse Anti-Trx-Tag antibody

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Thioredoxin; ADF; Surface associated sulphydryl protein; Thioredoxin-1; Thioredoxin 1; Thioredoxin1; TXN protein; ADF; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Surface associated sulphydryl protein; Surface-associated sulphydryl protein; TRDX; T

Cat:

SLM33018M

Species Reactivity：

Recombinant protein

Immunogen：

Recombinant human Thioredoxin

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Monoclonal

Isotype：

IgG

Applications：

WB=1:1000-5000ELISA=1:5000-10000not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Mouse

Product Overview：

Sample: Lane 1: Trx-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: SL33018P) at 4 ug Lane 2: Control (-) E.coli Lysate at 4 ug Primary: Anti-Thioredoxin/Trx (SLM33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kDSample: Lane 1: Recombined Trx/His-tag porcine GM-CSF protein (Aa18-144) at 2 ug Lane 2: Enzyme digested Trx-tag Primary: Anti-Trx-Tag (SLM33018M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 33/12 kD Observed band size: 33/12 kDSample: Lane 1: Trx-tagged fusion protein (32-186) (SL49104P) Overexpression E.coli Lysate at 2 ugLane 2: Trx-tagged fusion protein (19-213) (SL49106P) Overexpression E.coli Lysate at 2 ugLane 3: Trx-tagged fusion protein (20-152) (SL49107P) Overexpression E.coli Lysate at 2 ugPrimary: Anti-Trx-Tag (SLM33018M) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilutionPredicted band size: 30-40 kDObserved band size: 30-40 kD

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Unit:

Price: $196.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Function:
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

Subunit:
Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA-binding activity in a redox-dependent manner.

Subcellular Location:
Nucleus. Cytoplasm. Secreted. Note=Secreted by a leaderless secretory pathway. Predominantly in the cytoplasm in non irradiated cells. Radiation induces translocation of TRX from the cytoplasm to the nucleus.

Similarity:
Belongs to the thioredoxin family. Contains 1 thioredoxin domain.

SWISS:
P10599

Gene ID:
7295

Database links:

Entrez Gene: 7295 Human

Entrez Gene: 22166 Mouse

Entrez Gene: 116484 Rat

Omim: 187700 Human

SwissProt: P10599 Human

SwissProt: P10639 Mouse

SwissProt: P11232 Rat

Unigene: 435136 Human

Unigene: 260618 Mouse

Unigene: 29777 Rat

Picture

Sample:
Lane 1: Recombined Trx/His-tag porcine GM-CSF protein (Aa18-144) at 2 ug
Lane 2: Enzyme digested Trx-tag
Primary: Anti-Trx-Tag (SLM33018M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 33/12 kD
Observed band size: 33/12 kD

Sample:
Lane 1: Trx-tagged fusion protein (32-186) (SL49104P) Overexpression E.coli Lysate at 2 ug
Lane 2: Trx-tagged fusion protein (19-213) (SL49106P) Overexpression E.coli Lysate at 2 ug
Lane 3: Trx-tagged fusion protein (20-152) (SL49107P) Overexpression E.coli Lysate at 2 ug
Primary: Anti-Trx-Tag (SLM33018M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 30-40 kD
Observed band size: 30-40 kD

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