Nitric Oxide (NO) is a highly unstable biological free radical with small molecule, simple structure，NO
is gas at room temperature, slightly soluble in water and fat-soluble, which can diffuse through biofilms
quickly. As a new type of biological messenger molecule, it plays a role in transmitting signals between
cells and within cells. It is widely distributed in various tissues, especially neural tissues. It also plays a
very important role in the nervous, circulatory, respiratory, digestive, and urogenital systems of the body.
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NO is easily oxidized to form NO2 and NO3 in the body or in aqueous solution. This method uses nitrate
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reductase to reduce NO3 to NO2 specifically. Under acidic conditions, NO2 and Diazonium sulfonamide
produce diazo compounds. The compounds could further coupled with naphthyl vinyl diamine, the product
has a characteristic absorption peak at 550 nm, and its absorbance value can be measured to calculate the
NO content.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, low temperature centrifuge, water bath/constant temperature incubator, adjustable
pipette, 1mL glass cuvette, mortar/homogenizer, ice and distilled water, EP tube.
