Nitric Oxide (NO) is a highly unstable biological free radical with small molecule, simple structure，NO
is gas at room temperature, slightly soluble in water and fat-soluble, which can diffuse through biofilms
quickly. As a new type of biological messenger molecule, it plays a role in transmitting signals between
cells and within cells. It is widely distributed in various tissues, especially neural tissues. It also plays a
very important role in the nervous, circulatory, respiratory, digestive, and urogenital systems of the body.
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NO is easily oxidized to form NO2 and NO3 in the body or in aqueous solution. This method uses nitrate
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reductase to reduce NO3 to NO2 specifically. Under acidic conditions, NO2 and diazonium sulfonamide
produce diazo compounds. The compounds could further coupled with naphthyl vinyl diamine, the product
has a characteristic absorption peak at 550 nm, and its absorbance value can be measured to calculate the
NO content.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, low temperature centrifuge, water bath/constant temperature
incubator, adjustable pipette, micro glass cuvette/ 96-well flat-bottom plate, mortar/homogenizer, ice and
distilled water, EP tube.
