Hydrogen sulfide (H2S) is a new type of gaseous signal molecule. It is a neurotransmitter that exists in
the brain. The physiological concentration of H2S has an important regulatory effect on the long-term
enhancement of the hippocampus of the nervous system. It also plays an important pathophysiological
effect on the process of spontaneous hypertension, hemorrhagic shock and liver cirrhosis.
H2S can react with N, N-dimethyl-p-phenylenediamine and ferric ammonium sulfate to form methylene
blue. Methylene blue has a maximum absorption peak at 665nm. The H2S content can be calculated by
measuring the absorbance value.
Reagents and Equipment Required but Not Provided：
Spectrophotometer/microplate reader, desk centrifuge, pipette, micro glass cuvette/96 well flat-bottom
plate, mortar/homogenizer, ice and distilled water.
