Hg2+ is an important toxic heavy metal ion in water, which easily absorbed and accumulated by organisms
and can be further transmitted through the food chain, causing damage. Minamata disease is a kind of
typical mercury poisoning.
After digestion, Hg2+ can form one orange complex with Dithizone in acid environment, which can be
dissolved in chloroform and has a maximum absorption peak at 490 nm. In this kit, the content of Hg2+ is
quantified by measuring the color development at 490 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, centrifuge, adjusted transferpettor, 1 mL glass cuvette, concentrated sulfuric acid
(H2SO4), concentrated nitric acid (HNO3), distilled water.
