Phosphoenolpyruvate Carboxykinase(PEPCK) Activity Assay Kit

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磷酸烯醇式丙酮酸羧激酶（PEPCK）活性检测试剂盒

Cat:

NA0393

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 110 mL ×1. Storage at 4℃.

Reagent I: 18 mL×1, stored at 4℃.

Reagent II: Powder×1, stored at -20℃ and protected from light. Dissolve it in 15 mL of Reagent I before

use. The reagent that cannot be used up shall be stored at -20℃ after repacking, repeat freezing and

thawing are prohibited.

Reagent III: 18 μL×1, stored at 4℃ and protected from light. Before temporary use, add distilled water to

dilute according to the volume ratio of 1:120, prepare when the solution will be used.

Reagent IV: 62 μL×1, stored at 4℃ and protected from light. Before temporary use, add distilled water to

dilute according to the volume ratio of 7:250, prepare when the solution will be used.

Reagent V：Powder×1, stored at -20℃ and protected from light. Dissolve it in 2.5 mL of distilled water

before use. The reagent that cannot be used up shall be stored at -20℃ after repacking, repeated freezing

and thawing are prohibited.

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Unit:

Price: $528

Product PDFs

Datasheet:

PEPCK (EC 4.1.1.32) is widely found in animals, flowering plants, algae, some fungi and bacteria. The

enzyme catalyzes the conversion of oxaloacetic acid to phosphoenolpyruvate, which is the first-rate

limiting enzyme regulating gluconeogenesis.

PEPCK catalyzes oxaloacetic acid to form phosphoenolpyruvate and CO₂, pyruvate kinase and lactate

dehydrogenase further catalyze the oxidation of NADH to NAD⁺in turn, and determine the NADH decline

rate at 340 nm, which can reflect the PEPCK activity.

Reagents and Equipment Required but Not Provided

Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, water bath pot, micro quartz

cuvette/96 well flat-bottom plate (UV), adjustable pipette, mortar/homogenizer, ice and distilled water

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