Glycogen Phosphorylase a (Gpa) Activity Assay Kit

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糖原磷酸化酶a（GPa）活性检测试剂盒

Cat:

NA0390

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

-20℃

Product Overview：

Components:

Reagent

Extract solution

Reagent I

Size

Storage

4℃

110 mL×1

20 mL×1

Powder×1

Powder×2

4℃

Reagent II

4℃

Reagent III

Reagent IV

Reagent V

Reagent VI

4℃

-20℃

Solution preparation:

1. Reagent II: Dissolved with 0.5 mL of distilled water before use. Mix thoroughly.

2. Reagent III: Dissolved with 0.5 mL of distilled water before use. Mix thoroughly. It can be divided

into small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

3. Reagent IV: Dissolved with 1.25 mL of distilled water before use. Mix thoroughly. It can be divided

into small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

4. Reagent V: Dissolved with 1 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

5. Reagent VI: Dissolved with 1 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and stored for 4 weeks at -20℃. Avoid repeating freeze/thaw cycles.

6. Working solution: Calculate according to the amount required for the experiment before use, according

to the ratio of reagent I: reagent II: reagent III: reagent IV: distilled water= 148μL: 4μL: 4μL:

4μL :10μL(amount of 1T), mix well before use.

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Unit:

Price: $462

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Glycogen phosphorylase is divided into active glycogen phosphorylase a (Glycogen phosphorylase a, GPa)

and inactive glycogen phosphorylase b (Glycogen phosphorylase b, GPb) two forms. The decomposition of

glycogen is mainly carried out under the catalysis of glycogen phosphorylase a. When no activator is added,

glycogen phosphorylase a catalyzes the production of glucose residues from glycogen and inorganic

phosphorus to glycogen and glucose 1-phosphate. Under the action of phosphoglucose mutase and 6-

phosphate glucose dehydrogenase, it further catalyzes the reduction of NADP to NADPH. Measuring the

rate of increase of NADPH at 340nm can reflect the activity of glycogen phosphorylase a.

BC3345 -- page 1 / 3

Reagents and Equipment Required but Not Provided：

Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, constant temperature

incubator/water bath, adjustable pipette, mortar/homogenizer, micro quartz cuvette/96 well UV plate, ice

and distilled water.

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