Soil N-acetyl-β-D-glucosidase(S-NAG) is an acid hydrolase in lysosomes secreted by soil microorganisms.
The activity of S-NAG is closely related to some pathological condition of the body.
S-NAG can catalyze the 4-Nitrophenyl-N-acetyl-β-D-glucopyranoside to p-nitrophenol. The product has
characteristic of absorption at 400 nm. In this kit, the S-NAG activity is quantified by measuring the
increase in the color development at 400 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, water-bath, desk centrifuge, transferpettor, 1 mL glass cuvette, analytical balance,
mortar, 30 mesh sieve (or samller), ice and distilled water.
